1. 检查服务器->Add Or Remove Windows Features -> IIS 是否安装了Asp.Net 3.5 和Asp.net 4.5
2. 检查初始页面是否存在
3. 检查Application pool的.net版本
4. web config -> 中添加

<system.webServer>
    <handlers>
      <remove name="ExtensionlessUrlHandler-Integrated-4.0"/>
<add name="ExtensionlessUrlHandler-Integrated-4.0" path="*." verb="*" type="System.Web.Handlers.TransferRequestHandler" preCondition="integratedMode,runtimeVersionv4.0"/>
    </handlers>
<validation validateIntegratedModeConfiguration="false"/>
</system.webServer>

5. 检查Application pool的用户是否有访问虚拟目录的权限
6. 检查Route